A new method for the extraction and purification of K99 pili from enterotoxigenic Escherichia coli and their characterization

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A new method for the extraction and purification of K99 pili from enterotoxigenic Escherichia coli and their characterization.

It was found that K99 pili from enterotoxigenic Escherichia coli (of bovine origin) could be extracted by treatment with 3M-KSCN solution. The K99 pili were purified by preparative isoelectric focusing to apparent homogeneity as judged by the presence of a single band on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis; the molecular weight of this component was calculated to be 12 60...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1982

ISSN: 0264-6021

DOI: 10.1042/bj2010505